BACTERIOPHAGE-INDUCED MUTATION IN ESCHERICHIA COLI

Lysis of Escherichia coli cells induced by bacteriophage T4.

Structural changes in the envelope of Escherichia coli cells accompanying their lysis from without by bacteriophage T4 have been studied. The hypothesis concerning the role of collapse of membrane potential and formation of periplasmic vesicles in the process of lysis from without has been advanced.

Pasteurella Bacteriophage Sex Specific in Escherichia coli.

Phage H, thought to be specific for Pasteurella pestis, was shown to plate efficiently on F(-) strains of Escherichia coli but not on F(+), F', or Hfr strains. The phage was adsorbed rapidly to F(-) strains but was not adsorbed to strains carrying F. Comparison with seven other reported female-specific phages showed that, although phage H was similar to the other phages in some characteristics,...

Adaptive mutation in Escherichia coli.

In 1988 John Cairns, Julie Overbaugh, and Stephan Miller published a paper entitled “The origin of mutants,” in which they suggested that bacteria could choose which mutations to make (11). Shortly thereafter, Cairns and I began collaborating on a National Science Foundation-funded project to investigate the genetic basis of what was popularly called “directed mutation” (although at the time we...

Escherichia coli XL10-gold bacteria produce bacteriophage.

The Effect of Mutation and Selection on Codon Adaptation in Escherichia coli Bacteriophage

Studying phage codon adaptation is important not only for understanding the process of translation elongation, but also for reengineering phages for medical and industrial purposes. To evaluate the effect of mutation and selection on phage codon usage, we developed an index to measure selection imposed by host translation machinery, based on the difference in codon usage between all host genes ...